Review





Similar Products

accessory plasmid ap selection system papzif268  (Addgene inc)
93
Addgene inc accessory plasmid ap selection system papzif268
Accessory Plasmid Ap Selection System Papzif268, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accessory+plasmids/pm33134656__ao0c03508_si_001-10-2-13?v=Addgene+inc
Average 93 stars, based on 1 article reviews
accessory plasmid ap selection system papzif268 - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
accessory plasmid pjpc12 δps prmb0034 genevi  (Addgene inc)
93
Addgene inc accessory plasmid pjpc12 δps prmb0034 genevi
Accessory Plasmid Pjpc12 δps Prmb0034 Genevi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accessory+plasmids/pm39462751-184-13-16?v=Addgene+inc
Average 93 stars, based on 1 article reviews
accessory plasmid pjpc12 δps prmb0034 genevi - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
plasmids for the originally used constructs of accessory proteins  (Addgene inc)
90
Addgene inc plasmids for the originally used constructs of accessory proteins
Plasmids For The Originally Used Constructs Of Accessory Proteins, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accessory+plasmids/pm38969281-56-8-22?v=Addgene+inc
Average 90 stars, based on 1 article reviews
plasmids for the originally used constructs of accessory proteins - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
accessory protein expression vectors  (Addgene inc)
93
Addgene inc accessory protein expression vectors
SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
Accessory Protein Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accessory+plasmids/pmc11191075-143-3-10?v=Addgene+inc
Average 93 stars, based on 1 article reviews
accessory protein expression vectors - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
accessory plasmids  (Addgene inc)
93
Addgene inc accessory plasmids
SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
Accessory Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accessory+plasmids/pm38630589-290-60-65?v=Addgene+inc
Average 93 stars, based on 1 article reviews
accessory plasmids - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
plasmid encoding hiv 1 tat accessory protein  (Addgene inc)
94
Addgene inc plasmid encoding hiv 1 tat accessory protein
SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
Plasmid Encoding Hiv 1 Tat Accessory Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accessory+plasmids/pmc10973674-100-26-32?v=Addgene+inc
Average 94 stars, based on 1 article reviews
plasmid encoding hiv 1 tat accessory protein - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
plasmid encoding hiv 1 rev accessory protein  (Addgene inc)
94
Addgene inc plasmid encoding hiv 1 rev accessory protein
SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
Plasmid Encoding Hiv 1 Rev Accessory Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accessory+plasmids/pmc10973674-100-35-41?v=Addgene+inc
Average 94 stars, based on 1 article reviews
plasmid encoding hiv 1 rev accessory protein - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
accessory plasmid ptofa  (TaKaRa)
86
TaKaRa accessory plasmid ptofa
SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
Accessory Plasmid Ptofa, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accessory+plasmids/pm37944824-174-26-30?v=TaKaRa
Average 86 stars, based on 1 article reviews
accessory plasmid ptofa - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
accessory plasmid  (TaKaRa)
86
TaKaRa accessory plasmid
SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
Accessory Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accessory+plasmids/pm37832876-199-10-13?v=TaKaRa
Average 86 stars, based on 1 article reviews
accessory plasmid - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
accessory capsid plasmid pdp9  (PlasmidFactory gmbh)
90
PlasmidFactory gmbh accessory capsid plasmid pdp9
SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
Accessory Capsid Plasmid Pdp9, supplied by PlasmidFactory gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accessory+plasmids/pmc10905335-29-15-19?v=PlasmidFactory+gmbh
Average 90 stars, based on 1 article reviews
accessory capsid plasmid pdp9 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 expression. A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression vectors and then lysed for protein detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.

Journal: ACS Omega

Article Title: SARS-CoV-2 Protein Nsp9 Is Involved in Viral Evasion through Interactions with Innate Immune Pathways

doi: 10.1021/acsomega.4c02631

Figure Lengend Snippet: SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 expression. A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression vectors and then lysed for protein detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.

Article Snippet: SARS-CoV-2 nonstructural and accessory protein expression vectors were purchased from Addgene (MA, USA): pTwist-CMV-Nsp9–3xFlag (pGBW- m4133209), pTwist-CMV-ORF6–3xFlag (pGBW-m4252831), and pTwist-CMV-ORF3a- 3xFlag (pGBW-m4133902).

Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, Control